SMOBIO/[RP1300] ExcelRT™ Reverse Transcription Kit, 100 Rxn/s)"}]]> </ul> <a class=btn,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[RP1300] ExcelRT™ Reverse Transcription Kit, 100 Rxn/s)"}]]>

产品编号：RP1300

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Description

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.

Features

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity

Application

Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months

High yield

High sensitivity

Thermostable, up to 50°C, during first strand synthesis

Component

Volume

ExcelRT™ Reverse Transcriptase (200 U/μl)

100 μl

RNase Inhibitor (20 U/μl)

100 μl

5X RT Buffer (DTT)

500 μl

dNTP Mix (10 mM each)

200 μl

Oligo (dT)₂₀ (50 μM)

100 μl

Random Hexamers (100 μM)

100 μl

DEPC-TreatedH₂O

2 x 1 ml

Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol

5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 50 mM DTT, 375 mM KCl 15 mM MgCl2 and 50 mM DTT

Storage

-20°C for 24 months

Manual

Manual_RP1300_ExcelRT™ Reverse Transcription Kit

SDS

SDS_RP1300

What is the difference between ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400)?

ExcelRT™ Reverse Transcription Kits contain all components to synthesize high quality first strand cDNA. The components in ExcelRT™ Reverse Transcription Kit (RP1300) and ExcelRT™ Reverse Transcription Kit II (RP1400) are the same.

The components in RP1300 are separated. This make RP1300 more flexible to fit different experimental requirements.

Selected components in RP1400 are pre-mixed. This make RP1400 more convenient to use.

ExcelRT™ Reverse Transcription Kit (RP1300) contains Random hexamers and Oligo(dT)₂₀, how do I choose?

You can choose oligo d(T)₂₀, Random hexamers, or both depending on the RNA template and the downstream applications.

Oligo (dT)20 anneals to poly(A) tails of eukaryotic mRNAs. It is suitable for cDNA libraries construction from eukaryotic mRNAs, full- length cDNA cloning, and 3′ rapid amplification of cDNA ends (3′ RACE). It is not suitable for cDNA synthesis from degraded RNA or RNAs that lack poly(A) tails, such as prokaryotic RNAs and microRNAs.

Random hexamers anneals to any RNA species, including poly(A) tails of eukaryotic mRNAs, degraded RNA, prokaryotic RNA, and RNAs that lack poly(A) tails (rRNA, tRNA, non-coding RNAs, small RNAs, and etc.) It is suitable for gene expression analysis. It is not suitable for full- length cDNA synthesis.

Combine oligo (dT)₂₀ and Random hexamers anneal to any RNA species. Oligo (dT)₂₀ provides additional coverage of 3’ end of RNA templates. It is suitable for gene expression analysis. It is not suitable for full- length cDNA synthesis.

Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis via Suppression of C-C Chemokine Receptor Type 7 Expression

Pelin Kaya, Sang R. Lee, Young Ho Lee, Sun Woo Kwon, Hyun Yang, Hye Won Lee, and Eui-Ju Hong1,

Nutrients. 2019 Feb; 11(2): 410. Published online 2019 Feb 15. doi: 10.3390/nu11020410

PMCID: PMC6412318

Loss of endogenous estrogen increases cardiac toxicity by doxorubicin

Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju HongJournal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146

[RP1400] ExcelRT™ Reverse Transcription Kit II

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C Reduced RNase H ribonuclease activity
Suitable for real-time PCR

[TF1000] SMO-HiFi™ DNA Polymerase

5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate—10 seconds/kb
High fidelity— 70 times higher than Taq polymerase
Generates blunt end amplicon
Thermo-stable for more than 10 hrs at 95°C.

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

产品列表：

斯莫比	TP1000	ExcelTaq DNA聚合酶，5 U /μl，500 U x 1	500 U x 1
斯莫比	TK1000	ExcelTaq Klen-Taq DNA聚合酶，5 U /μl，500 U x 1	500 U x 1
斯莫比	TF1000	ExcelTaq SMO-HiFi DNA聚合酶，5 U /μl，500 U x 1	500 U x 1
斯莫比	TP1100	ExcelTaq 5X PCR预混液，200 Rxn	200 Rxn
斯莫比	TP1200	ExcelTaq 5X PCR主染料混合物，200 Rxn	200 Rxn
斯莫比	TP1260	ExcelTaq 5X荧光PCR预混液，200 Rxn	200 Rxn
斯莫比	TP2000	ExcelTaq血液直接DNA聚合酶，5 U /μl，500 U x 1	500 U x 1
斯莫比	TP2100	ExcelTaq 5X Blood Direct PCR Master Mix Kit，200 Rxn	200 Rxn
斯莫比	TQ1100	ExcelTaq Q-PCR预混液，200 Rxn	200 Rxn
斯莫比	CV1000	GetCloneTMPCR克隆载体，20 Rxn	200 Rxn
斯莫比	RP1000	ExcelRT反转录酶，20,000单位，100μl100 Rxn	100微升100 Rxn
斯莫比	RP1100	ExcelRT一步式RT-PCR试剂盒，50 Rxn	50 Rxn
Protein 相关产品
斯莫比	PM1500	ExcelBand全蓝色常规范围蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM1600	ExcelBand全蓝色常规范围加蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM1700	ExcelBand全蓝色广谱蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM2400	ExcelBand粉色蓝色蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM2500	ExcelBand 3色常规蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM2600	ExcelBand 3色高范围蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM2700	ExcelBand 3色广谱蛋白质标记物，250μlx 2	250微升×2
斯莫比	PM5000	ExcelBand 3色预染蛋白梯标准范围，250μlx 2	250微升×2
斯莫比	PM5100	ExcelBand 3色预染蛋白梯子高量程，250μlx 2	250微升×2
斯莫比	PM5200	ExcelBand 3色预染蛋白梯子，宽范围，250μlx 2	250微升×2
斯莫比	PS1000	FluoroStain蛋白荧光染色染料（红色，1000X），1毫升	1毫升
斯莫比	PS1001	FluoroStain蛋白荧光染色染料（红色，1000X），1 ml x 5	1毫升x 5
斯莫比	PS2000	FluoroStain蛋白荧光染色染料（金，100X），10毫升	10毫升
DNA 相关产品
斯莫比	DM1100	ExcelBand 50 bp DNA梯子，500μl	500微升
斯莫比	DM2100	ExcelBand 100 bp DNA梯子，500μl	500微升
斯莫比	DM2300	ExcelBand 100 bp + 3K DNA梯子，500μl	500微升
斯莫比	DM3100	ExcelBand 1KB（0.25-10 kb）DNA梯子，500μl	500微升
斯莫比	DM3200	ExcelBand 1KB Plus（0.1-10 kb）DNA梯子，500μl	500微升
斯莫比	DM4100	ExcelBand XL 25 kb DNA梯子，宽量程（最高25 kb），500μl	500微升
斯莫比	DM1160	FluoroBand 50 bp荧光DNA梯子，500μl	500微升
斯莫比	DM2160	FluoroBand 100 bp荧光DNA梯子，500μl	500微升
斯莫比	DM2360	FluoroBand 100 bp + 3K荧光DNA梯子，500μl	500微升
斯莫比	DM3160	FluoroBand 1KB（0.25-10 kb）荧光DNA梯子，500μl	500微升
斯莫比	DM3260	FluoroBand 1KB Plus（0.1-10 kb）荧光DNA梯子，500μl	500微升
斯莫比	DM4160	FluoroBand XL 25 kb荧光DNA梯子，宽量程（最大25 kb），500μl	500微升
斯莫比	DL1000	ExcelDye 6X DNA加载染料，橙色，5毫升x 2	5毫升x 2
斯莫比	DL2000	ExcelDye 6X DNA加载染料，绿色，5毫升x 2	5毫升x 2
斯莫比	DL3000	ExcelDye 6X DNA加载染料，蓝色，5毫升x 2	5毫升x 2
斯莫比	DL4000	ExcelDye 6X DNA加载染料，三色，5 ml x 2	5毫升x 2
斯莫比	DL5000	FluoroDye DNA荧光加载染料（绿色，6X），1毫升	1毫升
斯莫比	DL5001	FluoroDye DNA荧光加载染料（绿色，6X），1 ml x 5	1毫升x 5
斯莫比	DS1000	FluoroStain DNA荧光染色染料（绿色，10,000X），500μl	500微升
斯莫比	DS1001	FluoroStain DNA荧光染色染料（绿色，10,000X），500μlx 5	500微升x 5
LED Light Box 切胶仪
斯莫比	VE0100	B-BOXTM蓝光LED落射照明器，DC 12伏，0.72安培	1套
斯莫比	VE0101	适配器，AC 100-240V，50/60 Hz，1.8 m电源线，1 /包
斯莫比	VE0102	用于B-BOXTM的多角度滤板，1 /包
斯莫比	VE0103	用于B-BOXTM的琥珀色护目镜，1 /包