SMOBIO/[CC0202] Champion™ 109 High, 80 tubes/s)"}]]> </ul> <a class=btn btn-default bt,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[CC0202] Champion™ 109 High, 80 tubes/s)"}]]>

产品编号：CC0202

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>克隆和表达>克隆试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C.

Storage and expiration

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Genotypes and applications

Product Name

Genotype

Application

Champion™ 109 High

e14(McrA-)recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 thi-1 relA1 ∆(lac-proAB) (F’traD36 proAB lacIqZ∆M15

Appropriate for blue-white color and robotic screening. It is a fast growing strain forming visible colonies within 8~10 hours.

Champion™ 21

F’ ompT hsdSβ(rβ-mβ-) dcm gal λ (DE3)

Appropriate host for recombinant protein expression using T7-based expression vectors.

Champion™ DH5α High

recA1 endA1 gyrA96 hsdR17(rk-, mK+) phoA supE44 relA1 thi-1 ∆(lacZYA-argF)U 169 φ80 ∆(lacZ)M15 F’

Suitable for cloning with large plasmid and cDNA library construction, and also for blue-white colony selection.

Items and ordering information

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

>1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

>1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

>3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

Manual

Manual_Champion™ Competent Cell

SDS

SDS_Champion™ Competent Cell

Flyer

Champion™ Competent Cell

Protocol card

Will transformation efficiency be reduced when Champion™ competent cells are thawed, dispensed and refrozen repeatedly?

When Champion™ competent cells are thawed dispensed in aliquots and refrozen within 1 min, the transformation efficiency will be 10~20% reduced compared to the first time use.

What is the advantage of thawing competent cells with circulating water instead of still water?

Using circulating water can reduce the thawing time. Less thawing time shows higher efficiency than long thawing time.

Is there any difference of transformation efficiency between using plating beads, bending glass rod and plating loop?

The bending glass rod shows best efficiency and the plating loop show less efficiency than other method. For handle a lot samples at the same time the plating beads are recommended.

Is 1 second vortex critical for mixture the DNA?

One second vortex provides more reliable transformation efficiency (1.1 times compare with mixed by finger tapping)

Will heat shock affect the transformation efficiency?

Heat shock treatment will enhance the efficiency about 1~2X versus non-heat shock method.

Thawing methods

Shorter thawing time is more efficient than a longer thawing time. Slow thawing caused by power shortages and unstable freezers will result in decreased efficiency.

Size of plasmid

Plasmid size affect the efficiency greatly. The efficiency of transforming a supercoiled 2.7 kb is approximately 100 times higher than that of a 10 Kb plasmid (using time-saving transformation protocol). For large plasmids (> 6 kb), standard transformation protocol is recommended.

Heat shock treatment

Heat shock treatment will enhance the efficiency about 1~2 folds versus non-heat shock method.

Plating methods

Bent glass rods show the greatest efficiency, while plating loop shows less efficiency than plating beads. When handling a large quantity of samples at the same time, plating beads are recommended.

Concentration of antibiotic

Antibiotic concentration is critical to use of the time-saving transformation protocol.

Antibiotic

Concentration

Ampicillin (Ap)

20 μg/ml

Kanamycin (Km)

25 μg/ml

Tetracycline (Tc)

7.5 μg/ml

Chloramphenicol (Cm)

20 μg/ml

For plasmid size <6 Kb, the efficiency of kanamycin selection is usually 3~10 times less than the ampicillin selection. For plasmid size > 6 Kb, the efficiency of kanamycin selection is much lower than ampicillin. We suggest using the standard transformation protocol (with heat shock and recovery steps) to enhance the efficiency.

Product Name

Compatible to E. coli strain

Efficiency (cfu/μg)

Quantity

Cat. No.

Champion™ 109 High

E. coli JM109

>1 x 108

100 μl x 80 vials

CC0202

100 μl x 24 vials

CC0204

Champion™ 21

E. coli BL21 (DE3)

>1 x 107

100 μl x 80 vials

CC2102

100 μl x 24 vials

CC2104

Champion™ DH5α High

E. coli DH5α

>3 x 108

100 μl x 80 vials

CC5202

100 μl x 24 vials

CC5204

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)
[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.

Safe fluorescent dyes

[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

Blue-light illuminator

[VE0100] B-BOX™ Blue Light LED Epi-illuminator

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.

[CV1000] GetClone™ PCR Cloning Vector, 20 Rxn
[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn

Colony PCR

Analyze colonies with PCR master mix to save preparation time.

[TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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