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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
SMOBIO/[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 Rxn/SYBR, no ROX), 200 Rxn
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗小鼠>
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SMOBIO/[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 Rxn/SYBR, no ROX), 200 Rxn
商品介绍

 

Description 

The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system. 

Features

  • High sensitivity and signal intensity

  • Smart blue contrast dye as a visual aid for reaction setup

  • Better compatibility for reverse transcription

  • Low background 

Storage 

Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months

Odoo - Sample 1 for three columns

Sensitivity

The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.

Odoo - Sample 2 for three columns

Better Compatibility for reverse transcription 

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.

Odoo - Sample 3 for three columns

Specificity 

The standard curve (right) and melting curve (left)  of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).

Contents

Component

Volume

Cat. No

ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX)

2 x1 ml

TQ1100 (200 Rxn)

ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX)

5 x1 ml

TQ1101 (500 Rxn)

Storage 

Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months

 

Manual

Manual_TQ1100_ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

SDS

SDS_TQ1100

Flyer

SMOBIO’s Q-PCR Master Mix- Instrument Compatibility

 

 

Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore

Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y

PMCID: PMC6757040

Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328

Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells 

Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

PMCID: PMC5522218

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver 

Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

PMCID: PMC5706436

Loss of endogenous estrogen increases cardiac toxicity by doxorubicin

Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong

Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146

ExcelTaq™ 2X Q-PCR Master Mix

Product Name

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

Blue contrast dye for best convenience

ü

ü

ü

ü

X

ROX dye for reference

X

ü

X

ü

ü

Time required for enzyme activation

10min

10min

2min

2min

10min

qPCR program

Standard

Standard

Fastand Standard

Fastand Standard

Standard

品牌介绍

SMOBIO


SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。


2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。


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