Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.
The standard curve (right) and melting curve (left) of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).
Component | Volume | Cat. No |
ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX) | 2 x1 ml | TQ1100 (200 Rxn) |
ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX) | 5 x1 ml | TQ1101 (500 Rxn) |
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Manual
Manual_TQ1100_ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)
SDS
SDS_TQ1100
Flyer
SMOBIO’s Q-PCR Master Mix- Instrument Compatibility
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
Instrument Compatibility* | |||||
Applied Biosystems system: | |||||
5700, 7000, 7300, 7700, and 7900HT | Δ | V | Δ | V | V |
StepOne, StepOnePlus | Δ | V | Δ | V | V |
7500, 7500 Fast, QuantStudio, ViiA7 | Δ | Δ | Δ | Δ | Δ |
BioRad system: | |||||
CFX96 , CFX384 | V | V | V | V | V |
iQ5 | V | V | V | V | V |
Opticon, Opticon 2, Chromo 4 | V | V | V | V | V |
Cepheid system: | |||||
Smart Cycler | V | V | V | V | V |
Eppendorf system: | |||||
Mastercycler ep realplex | V | V | V | V | V |
Roche system: | |||||
LightCycler 480, Nano | V | V | V | V | V |
QIAgen system: | |||||
Rotor-Gene™ 3000, 6000, Q | V | V | V | V | V |
Agilent (Stratagene) system: | |||||
MX3000P, 3005P, 4000P | Δ | Δ | Δ | Δ | Δ |
Illumina system: | |||||
Eco | V | V | V | V | V |
Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)
Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore
Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y
PMCID: PMC6757040
Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.
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Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
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Loss of endogenous estrogen increases cardiac toxicity by doxorubicin
Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
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Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10min | 10min | 2min | 2min | 10min |
qPCR program | Standard | Standard | Fastand Standard | Fastand Standard | Standard |
SMOBIO
SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。
产品列表:
斯莫比 | TP1000 | ExcelTaq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物,200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit,200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体,20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶,20,000单位,100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒,50 Rxn | 50 Rxn |
Protein 相关产品 | |||
斯莫比 | PM1500 | ExcelBand全蓝色常规范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1600 | ExcelBand全蓝色常规范围加蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围,250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程,250μlx 2 | 250微升×2 |
斯莫比 | PM5200 | ExcelBand 3色预染蛋白梯子,宽范围,250μlx 2 | 250微升×2 |
斯莫比 | PS1000 | FluoroStain蛋白荧光染色染料(红色,1000X),1毫升 | 1毫升 |
斯莫比 | PS1001 | FluoroStain蛋白荧光染色染料(红色,1000X),1 ml x 5 | 1毫升x 5 |
斯莫比 | PS2000 | FluoroStain蛋白荧光染色染料(金,100X),10毫升 | 10毫升 |
DNA 相关产品 | |||
斯莫比 | DM1100 | ExcelBand 50 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2100 | ExcelBand 100 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子,500μl | 500微升 |
斯莫比 | DM3100 | ExcelBand 1KB(0.25-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM3200 | ExcelBand 1KB Plus(0.1-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子,宽量程(最高25 kb),500μl | 500微升 |
斯莫比 | DM1160 | FluoroBand 50 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2160 | FluoroBand 100 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB(0.25-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus(0.1-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子,宽量程(最大25 kb),500μl | 500微升 |
斯莫比 | DL1000 | ExcelDye 6X DNA加载染料,橙色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL2000 | ExcelDye 6X DNA加载染料,绿色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料,蓝色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料,三色,5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料(绿色,6X),1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料(绿色,6X),1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
斯莫比 | VE0100 | B-BOXTM蓝光LED落射照明器,DC 12伏,0.72安培 | 1套 |
斯莫比 | VE0101 | 适配器,AC 100-240V,50/60 Hz,1.8 m电源线,1 /包 | |
斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板,1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜,1 /包 |