SMOBIO/[TP5000] ExcelTaq™ Hot Start II DNA Polymerase (5 U/μl, 500 U)/5 U/μl, 500 U/TP5000,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[TP5000] ExcelTaq™ Hot Start II DNA Polymerase (5 U/μl, 500 U)/5 U/μl, 500 U/TP5000

产品编号：TP5000

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>其它试剂盒>定量试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Product overview
Technical/Spec
Support/Documents
Tips for usage
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Description

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Features

Aptamer-based hot start PCR
Reversible enzyme inactivation
Omits extra enzyme activation step
Convenient for room temperature PCR set-up
High yield and specificity of target amplicons
Wide range of amplicon length (up to 10 kb)
High sensitivity (as low as 1 fg of plasmid)

Applications

High specificity PCR
High-throughput PCR
Generation of PCR products for TA cloning
Routine PCR, multiplex PCR, colony PCR, and RT-PCR

Storage

-20°C for 24 months

Hot Start

ExcelTaq™ Hot Start II shows absolute amplicon when pre-incubation at 37˚C and 42˚C.

Reactions were subjected to a pre-incubation at 37˚C or 42˚C for 10 minutes before initial denaturation step. General Taq DNA polymerase generated primer dimers and failed to amplify target due to enzyme activated at 37˚C or 42˚C.

High specificity

ExcelTaq™ Hot Start II DNA Polymerase shows high specificity on amplifying target DNA.

The optimal annealing temperature of GAPDH primer set is 58˚C. Improper annealing temperature set at 52˚C may force primer-dimer formation. ExcelTaq™ Hot Start II DNA Polymerase eliminated primer-dimer and increased amounts of desired product. Taq DNA polymerase reduced target amplification due to primer-dimer formation at improper annealing temperature of 52˚C.

High sensitivity

ExcelTaq™ Hot Start II DNA Polymerase shows high sensitivity to amplify from low amount of templates.

Each set of PCR reactions contained either 1 pg, 10 pg, or 1 ng of HeLa cell cDNA as templates. ExcelTaq™ Hot Start II DNA Polymerase successfully amplified targets from lower amount of templates, in comparison with hot-start DNA polymerases from other suppliers and general Taq DNA polymerase.

High sensitivity

ExcelTaq™ Hot Start II DNA Polymerase amplifies from plasmid template amounts as low as 1 fg.

Component

Volume

Hot start II DNA Polymerase (5 U/μl)

100 μl

10X HS Buffer

2 x 1 ml

Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol

10X HS buffer

200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage

-20°C for 24 months

Manual

Manual_TP5000_ExcelTaq™ Hot Start II DNA Polymerase

SDS

SDS_TP5000

Flyer

ExcelTaq™ Hot Start II DNA Polymerase

Recommended PCR Condition

Template

1– 150 ng*

Forward primer

0.1– 0.5 µM

Reverse primer

0.1– 0.5 µM

10X HS Buffer

5µl

dNTPs

0.2 mM (each)

Hot Start II DNA Polymerase

0.25µl (1.25U)

H₂O

to50 µl

Total volume

50µl

*Optimal amount of DNA template depends on the source and quality of DNA. The amount of purified plasmid templates can be even less than 1 pg.

Recommended PCR Program

Steps

Temp.

Time

Cycles

Templatedenature

94°C

2 min

Denature

94°C

30 sec

25-40

Annealing

50-68°C**

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

**Optimal PCR condition variesaccording to primers’ thermodynamic properties.

[RP1000] ExcelRT™ Reverse Transcriptase

High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity

[TP1000] ExcelTaq™ Taq DNA Polymerase

5"→3" DNA polymerase activity
5"→3" exonuclease activity
None detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3’-dA overhangs
Thermo-stable-half life is more than 40 min at 95°C

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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