Description
ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.
Features
5"→3" DNA polymerase activity
5"→3" exonuclease activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3’-dA overhangs
Thermo-stable – half-life lasts for more than 40 min at 95°C
Applications
Routine PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling
Storage
-20°C for 24 months
ExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).
ExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).
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Storage Buffer
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
10X Taq buffer
200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C for 24 months
Manual
Manual_TP1000_ExcelTaq™ Taq DNA Polymerase
SDS
SDS_TP1000
Recommended PCR Condition
Template | 1 – 150 ng |
Forward primer | 0.1 – 0.5 µM |
Reverse primer | 0.1 – 0.5 µM |
10X Taq Buffer | 5 µl |
dNTPs | 0.2 mM (each) |
Taq DNA Polymerase | 0.25 µl (1.25U) |
H2O | to 50 µl |
Total volume | 50 µl |
Recommended PCR Program
Steps | Temp. | Time | Cycles |
Template denature | 94°C | 2 min | 1 |
Denature | 94°C | 30 sec | 25-40 |
Annealing | 50-68°C* | 30 sec | |
Extension | 72°C | 30 sec/kb | |
Final extension | 72°C | 1 min | 1 |
*Optimal PCR condition varies according to primers’ thermodynamic properties.
Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
Aptamer-based hot start PCR
Reversible enzyme inactivation
Omits extra enzyme activation step
Convenient for room temperature PCR set-up
High yield and specificity of target amplicons
Wide range of amplicon length (up to 10 kb)
High sensitivity (as low as 1 fg of plasmid)
High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye
5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
SMOBIO
SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。
产品列表:
斯莫比 | TP1000 | ExcelTaq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物,200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit,200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体,20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶,20,000单位,100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒,50 Rxn | 50 Rxn |
Protein 相关产品 | |||
斯莫比 | PM1500 | ExcelBand全蓝色常规范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1600 | ExcelBand全蓝色常规范围加蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围,250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程,250μlx 2 | 250微升×2 |
斯莫比 | PM5200 | ExcelBand 3色预染蛋白梯子,宽范围,250μlx 2 | 250微升×2 |
斯莫比 | PS1000 | FluoroStain蛋白荧光染色染料(红色,1000X),1毫升 | 1毫升 |
斯莫比 | PS1001 | FluoroStain蛋白荧光染色染料(红色,1000X),1 ml x 5 | 1毫升x 5 |
斯莫比 | PS2000 | FluoroStain蛋白荧光染色染料(金,100X),10毫升 | 10毫升 |
DNA 相关产品 | |||
斯莫比 | DM1100 | ExcelBand 50 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2100 | ExcelBand 100 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子,500μl | 500微升 |
斯莫比 | DM3100 | ExcelBand 1KB(0.25-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM3200 | ExcelBand 1KB Plus(0.1-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子,宽量程(最高25 kb),500μl | 500微升 |
斯莫比 | DM1160 | FluoroBand 50 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2160 | FluoroBand 100 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB(0.25-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus(0.1-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子,宽量程(最大25 kb),500μl | 500微升 |
斯莫比 | DL1000 | ExcelDye 6X DNA加载染料,橙色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL2000 | ExcelDye 6X DNA加载染料,绿色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料,蓝色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料,三色,5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料(绿色,6X),1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料(绿色,6X),1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
斯莫比 | VE0100 | B-BOXTM蓝光LED落射照明器,DC 12伏,0.72安培 | 1套 |
斯莫比 | VE0101 | 适配器,AC 100-240V,50/60 Hz,1.8 m电源线,1 /包 | |
斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板,1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜,1 /包 |