SMOBIO/[DM5100] ExcelBand™ Super Range DNA Ladder (50 bp-25 kb), 500 μl/500 μl/DM5100,SMOBIO,,SMOBIO,+,,Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[DM5100] ExcelBand™ Super Range DNA Ladder (50 bp-25 kb), 500 μl/500 μl/DM5100

产品编号：DM5100

市场价：¥0.00

场地：美国(厂家直采)

产品分类：其它>配件>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Description

The DM5100 ExcelBand™ Super Range DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM5100 is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are added for real time monitoring.

Features

Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months

Source

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range

50 ~ 25,000 bp

Concentration

88.7 µg/ 500 µl

Recommended loading volume

5 µl/ well

Storage

Room temperature for 6 months4°C for 12 months -20°C for 36 months

Specification

Cat. No.	DM5100
Series Name	ExcelBand™
Product Size	500 μl
Size Range	50 – 25000 bp
Band Number	26
Tracking Dye	Orange G, Bromophenol blue and Xylene cyanol FF
Enhanced Band	200, 500, 1200 and 3000 bp

Manual

Manual_DM5100_ExcelBand™ Super Range DNA Ladder (50 bp-25 kb)

SDS

SDS_DM5100

Are the DNA markers/ladders produced by SMOBIO sufficient in quantity?

Yes, all the DNA markers of SMOBIO have been passed in the QC processes including repeated optical density measurements to ensure the quantity of total DNA.

Are the SMOBIO"s DNA markers/ladders compatible to radio-labeling (for example, label DNA with T4 Polynucleotide Kinase)?

We do not recommend to do the labeling reaction directly.

The reasons are as follows:

Our DNA marker is pre-mixed with loading buffer that contains Tris-HCl, EDTA, and glycerol, may affect radio-labeling.
Our DNA marker is a mixture of PCR products and restriction enzyme digested DNA fragments. Digested dsDNA may still have phospho-group on their 5" end.

To enhance the efficiency of the labeling reaction, here are two steps suggested being done prior to the labeling reaction:

Purify DNA marker by EtOH precipitation or DNA purification kits to remove loading buffer.
Remove phospho-group by using phosphatase, ex CIAP (Calf intestinal alkaline phosphatase)

Are SMOBIO"s DNA markers/ladders suitable to use in DNA PAGE?

SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.

Why DNA markers/ladders are resolved two bands at the same size when using high percentage agarose or polyacrylamide gel?

DNA fragments with identical in size are indistinguishable on agarose gels, but, on acrylamide gels, even slight differences in DNA sequence can lead to noticeably different migration rates. To provide increased intensity of DNA marker/ladder bands, multiple DNA fragments with identical in size but different in DNA sequences are used. SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are more suitable for DNA PAGE.

Are SMOBIO"s DNA markers/ladders suitable to use in denaturing DNA PAGE?

SMOBIO"s AccuBand™ series DNA markers (DM1200, DM2000, DM2200 and DM2400) are suitable for DNA PAGE.

SMOBIO"s AccuBand™ DNA markers are not at denatured form, therefore, you need to denature DNA ladder by yourself.

Here is the protocol to denature DNA ladder:

Mix 5 μL of DNA Ladder with an equal volume of denaturing solution [95% (v/v) formamide, 10 mM EDTA (pH 8.0), 0.1% (w/v) bromophenol blue, 0.1% (w/v) xylene cyanol].
Incubate at 70°C for 5 minutes.
Electrophorese the sample in a denaturing polyacrylamide/urea gel

The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263

Transposable elements generate population-specific insertional patterns and allelic variation in genes of wild emmer wheat (Triticum turgidum ssp. dicoccoides)

Katherine Domb, Danielle Keidar, Beery Yaakov, Vadim Khasdan, Khalil Kashkush BMC Plant Biol. 2017; 17: 175. Published online 2017 Oct 27. doi: 10.1186/s12870-017-1134-z

PMCID: PMC5659041

ExcelBand™ DNA Ladder series

FluoroBand™ DNA Ladder series

AccuBand™ DNA Marker series

FluoroVue™ Nucleic Acid Gel Stain

Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light

FluoroStain™ DNA Fluorescent Staining Dye

Excellent for post staining
Sensitivity up to 0.04 ng DNA
A safe alternative to EtBr
Suitable for blue or UV light

FluoroDye™ DNA Fluorescent Loading Dye

Excellent for premix with DNA sample
Sensitivity up to 0.14 ng DNA
Safety dye
Convenience - monitor the electrophoresis in real-time

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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