![SMOBIO/[WM1000] YesBlot™ Western Marker I, 250 μl/250 μl/WM1000](images/SMOBIO/image.jpg)
Description
YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG-binding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Features
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 prestained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
Contents
The YesBlot™ Western Marker I contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer.
Storage
4°C for 3 months-20°C for 24 months
Specification
Cat. No. | WM1000 |
Series Name | YesBlot™ |
Product Size | 250 μl |
MW Range (Western Bands) | 15 – 200 kDa |
MW Range (Pre-stained Bands) | 10 – 70 kDa |
Band Number (Western Bands) | 10 |
Band Number (Pre-stained Bands) | 4 |
Pre-stained Band Color | Pink/Green/Blue |
Technical information
Principle of YesBlot™ Western Marker I
Manual
Manual_WM1000_YesBlot™ Western Marker I
SDS
SDS_WM1000
Flyer
Western Marker- Powerful for Western Data Credibility
Migration patterns and approximate MWs (kDa) of pre-stained bands
What is the composition of the YesBlot™ Western Marker I?
YesBlot™ Western Marker I contains ten IgG-binding proteins that binds the primary or secondary IgG antibody used to detect target protein, allowing you to detect the YesBlot™ Western Marker I directly. Addition to IgG-binding proteins, YesBlot™ Western Marker I contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size.
Does YesBlot™ Western Marker I selectively bind to IgG antibodies generated from different host species?
YesBlot™ Western Marker I can bind to IgG antibodies generated from mammalian host species, such as Human, Horse, Cow, Pig, Rat, Mouse, Rabbit, Goat, Sheep, Hamster, Guinea Pig, and etc.
Can YesBlot™ Western Marker I be used for protein quantification?
Since Western blot is a series of signal amplification, different kinds of primary antibodies and secondary antibodies will lead to different signal intensity on the film. We do not recommend using YesBlot™ Western Marker I for quantification purpose.
Will the YesBlot™ Western Marker I be detected using IgM antibodies?
YesBlot™ Western Marker I contains IgG binding proteins, which will not bind to IgM antibodies.
Can YesBlot™ Western Marker I be used with fluorescent-dye conjugated secondary antibody?
YesBlot™ Western Marker I can bind to IgG antibodies labeled with fluorescent dye.
ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
Q-PAGE™ Precast Gels
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
SMOBIO
SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。
产品列表：
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斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物，200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit，200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体，20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶，20,000单位，100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒，50 Rxn | 50 Rxn |
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斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围，250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程，250μlx 2 | 250微升×2 |
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DNA 相关产品 | |||
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斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子，500μl | 500微升 |
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斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB（0.25-10 kb）荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus（0.1-10 kb）荧光DNA梯子，500μl | 500微升 |
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斯莫比 | DL2000 | ExcelDye 6X DNA加载染料，绿色，5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料，蓝色，5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料，三色，5 ml x 2 | 5毫升x 2 |
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斯莫比 | DL5001 | FluoroDye DNA荧光加载染料（绿色，6X），1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料（绿色，10,000X），500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料（绿色，10,000X），500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
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![SMOBIO/[QP2320] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 12%), 10 gels/Mini, 15 wells, 12%), 10 gels</span>
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