Description
Ultrapure dUTP (2´-Deoxyuridine, 5´-Triphosphate) supplied as sodium salt in purified water (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
DNA amplification
Avoid carryover contamination between PCRs to eliminate a source of false positives.
Storage
-20°C for 24 months
Greater than 99% purity confirmed by HPLC
The purity of SMOBIO’s dNTP remained greater than 99% after one week storage at 25°C.
Product Name | dATP Solution - Sodium Salt | dTTP Solution - Sodium Salt | dCTP Solution - Sodium Salt | dGTP Solution - Sodium Salt | dUTP Solution - Sodium Salt |
Cat. No. | CD3000 | CD4000 | CD5000 | CD6000 | CD7000 |
Nomenclature | 2’-Deoxyadenosine 5’-triphosphate | 2’-Deoxythymidine 5’-triphosphate | 2’-Deoxycytidine 5’-triphosphate | 2’-Deoxyguanosine 5’-triphosphate | 2’-Deoxyuridine 5’-triphosphate |
Formula (Anion) | C10H13N5O12P3 | C10H14N2O14P3 | C9H13N3O13P3 | C10H13N5O13P3 | C9H12N2O14P3 |
Storage | at -20 °C | at -20 °C | at -20 °C | at -20 °C | at -20 °C |
Stability | 24 months | 24 months | 24 months | 24 months | 24 months |
Appearance | Colorless | Colorless | Colorless | Colorless | Colorless |
Concentration | 100-110mM | 100-110mM | 100-110mM | 100-110mM | 100-110mM |
Purity (HPLC) | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% |
A250/A260 | 0.78±0.02 | 0.64±0.02 | 0.82±0.02 | 1.15±0.03 | 0.74±0.03 |
A280/A260 | 0.15±0.01 | 0.74±0.02 | 0.97±0.02 | 0.67±0.02 | 0.38±0.02 |
A290/A260 | N/A | 0.24±0.02 | 0.30±0.02 | 0.28±0.02 | N/A |
pH (4°C) | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 |
Cation (Mg2+) | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM |
Phosphate (PO43-) | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM |
Contamination with human DNA (18S rRNA) or bacterial DNA (16S rRNA gene) | Negative | Negative | Negative | Negative | Negative |
Proteases, DNases, RNases, or Nicking Activity | Negative | Negative | Negative | Negative | Negative |
Manual
Manual_CD7000_SMOChem™ dUTP Solution - Sodium Salt
SDS
SDS_CD7000
Flyer
Purity of dNTPs- A Critical Factor for Successful PCR
SMOBIO"s dNTPs- Specification, Purity and Functionality
Preparation Note
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (20 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 20 μl dUTP to 50 μl water, PCR grade).
Product Description | Size | Cat. No. |
dATP Solution - Sodium Salt (100 mM) | 25 ml | CD3000 |
100 ml | CD3001 | |
dTTP Solution - Sodium Salt (100 mM) | 25 ml | CD4000 |
100 ml | CD4001 | |
dCTP Solution - Sodium Salt (100 mM) | 25 ml | CD5000 |
100 ml | CD5001 | |
dGTP Solution - Sodium Salt (100 mM) | 25 ml | CD6000 |
100 ml | CD6001 | |
dUTP Solution - Sodium Salt (100 mM) | 25 ml | CD7000 |
100 ml | CD7001 |
Deoxynucleotide (dNTP) Mix, 10 mM each (40 mM total) | 200 µl | CD1010 |
200 µl x 5 | CD1011 |
Deoxynucleotide (dNTP) Mix, 25 mM each (100 mM total) | 500 µl | CD1020 |
500 µl x 6 | CD1021 |
SMOBIO
SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。
产品列表:
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斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物,200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit,200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体,20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶,20,000单位,100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒,50 Rxn | 50 Rxn |
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斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围,250μlx 2 | 250微升×2 |
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DNA 相关产品 | |||
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斯莫比 | DM3200 | ExcelBand 1KB Plus(0.1-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子,宽量程(最高25 kb),500μl | 500微升 |
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斯莫比 | DM3260 | FluoroBand 1KB Plus(0.1-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子,宽量程(最大25 kb),500μl | 500微升 |
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斯莫比 | DL2000 | ExcelDye 6X DNA加载染料,绿色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料,蓝色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料,三色,5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料(绿色,6X),1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料(绿色,6X),1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μlx 5 | 500微升x 5 |
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斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板,1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜,1 /包 |