SMOBIO/[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX), 200 Rxn/TaqMan, ROX), 200 Rxn</span> </li> </ol> </div> <div class=col-sm-3 mb8> <form method=get action=/shop/category/cd,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[RQ2110] ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX), 200 Rxn/TaqMan, ROX), 200 Rxn

产品编号：RQ2110

市场价：¥0.00

场地：美国(厂家直采)

产品分类：试剂盒>PCR相关试剂盒>逆转录试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Product overview
Technical/Spec
Support/Documents
Tips for usage
Related products

Description

The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step qPCR Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step qPCR Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.

Features

High yield
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With ROX reference dye

Storage

Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)

Protect from light

-20°C for 12 months

Reverse transcription at wide template range

ExcelRT™ One-Step RT-qPCR Kit can quantitative analyze target RNA from a wide range of RNA template input. The amplification plot of one-step RT-qPCR with total RNA templates ranging from 1 pg to 1 ng in quantity, analyzed by using RQ2110 ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX) for RT-qPCR amplification.

Reverse transcription at wide temperature range (42-60°C)

ExcelRT™ One-Step RT-qPCR Kit can quantitative analyze target RNA at a wide temperature range (42-60°C). The amplification plot of one-step RT-qPCR with reverse transcription at temperature range from 42 to 60°C, analyzed by using RQ2110 ExcelRT™ One-Step RT-qPCR Kit (A) or kit from brand N (B) for RT-qPCR amplification. The overlapped amplification curves display that ExcelRT™ One-Step RT-qPCR Kit preforms successfully cDNA synthesis at wide temperature range.

Component

Volume

One-Step RT Enzyme Mix

400 μl

2X One-Step qPCR Master Mix

2 x1 ml

Storage

Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)

Protect from light

-20°for 12 months

Manual

Manual_RQ2110_ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)

SDS

SDS_RQ2110

Recommended primer design

Amplicon size: 80-150 bp
Tm value: around 60°C (calculated with Primer3 software)
Primer length: 17-25 mer
Sequence:

‒ 45-55% of GC content isrecommended.

‒ Avoid regional high GC or ATcontent

‒ Avoid palindrome sequence

‒ Sequence with G or C at the 3" end is recommended.

Specificityof primers should be confirmed through a BLAST search.

Recommended probe design

Tm value: 6-10°C higher than primers
Probe length: 20-30 mer
Sequence:

‒ 35-65% of GC content is recommended.

‒ Avoid regional high GC or AT content

‒ Select the strand contains more C’s than G’s

‒ Avoid palindrome sequence

‒ Avoid a G at the 5" end to prevent quenching of the 5’fluorophore.

Specificityof probe should be confirmed through a BLAST search.

Recommended reaction mixture set up for qPCR

Component

Volume

Final concentration

Template RNA

Varied

1 pg – 1 μg

Forward primer (10 μM)

Varied

125 – 900 nM

Reverse primer (10 μM)

Varied

125 – 900 nM

TaqMan Probe (10 μM)

Varied

125 – 900 nM

One-Step RT Enzyme Mix

2 μl

2X One-Step Master Mix

10 μl

ddH₂O

to 20 μl

Total volume

20 µl

*Template amount varies depending on the copy number of target present in the template solution.

** The PCR primer and probe concentration for an optimalqPCR reaction may vary according to primers’ and probe’s properties.

Recommended qPCRProgram

Standard program

Steps

Temp.

Time

Cycles

Reverse transcription

42°C - 60°C (45°C - 55°C is recommended)

10min

Enzyme activation

95°C

3 min

Denaturation

95°C**

15 sec

40-50

Annealing/ Extension

60°C

1 min

Fast program

Steps

Temp.

Time

Cycles

Reverse transcription

42°C - 60°C (45°C - 55°C is recommended)

5 min

Enzyme activation

95°C

20 sec

Denaturation

95°C**

3 sec

40-50

Annealing/ Extension

60°C

30 sec

[RP1000] ExcelRT™ Reverse Transcriptase

High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity

[RI1000] RNAok™ RNase Inhibitor

Application

RT-PCR
cDNA Synthesis
in vitro transcription

[RP1400] ExcelRT™ Reverse Transcription Kit II

Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR

[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

High sensitivity
High specificity
With ROX reference dye

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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