Description
The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Features
5"→3" DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High throughput PCR
High yield PCR
High reproducibility, with reduced pipetting errors
Compatible with most anticoagulants
Applications
Direct amplification of DNA from blood samples
High throughput screening without DNA purification
Suitable for multiplex PCR
Storage
Caution: Avoid Multiple Freeze/Thaw Cycles
4°C for 6 months-20°C for 24 months
ExcelTaq™ 5X Blood Direct PCR Master Mix amplified 200 bp from differently treated blood using CCR5 specific primers. (M: DM2100)
Component | Volume |
5X Blood Direct PCR Master Mix | 2 x 1 ml |
Positive Control Primers (10 μM,each) | 25 μl |
CCR5 control primer sequence
CCR5-F:5"-CTCCCAGGAATCATCTTTACC-3"
CCR5-R:5"-TCATTTCGACACCGAAGCAG-3"
Storage
Caution: Avoid Multiple Freeze/Thaw Cycles
4°C for 6 months-20°C for 24 months
Manual
Manual_TP2100_ExcelTaq™ 5X Blood Direct PCR Master Mix Kit
SDS
SDS_TP2100
Recommended PCR Condition
Blood | 3μl |
Forward primer | 0.1– 0.5 µM |
Reverse primer | 0.1– 0.5 µM |
5X Blood Direct PCR Master Mix | 10μl |
H2O | to50 μl |
Total volume | 50µl |
Note: Mix well before PCR Reaction
Recommended PCRProgram
Steps | Temp. | Time | Cycles |
Templatedenature | 94°C | 3 min | 1 |
Denature | 94°C | 30 sec | 25-40 |
Annealing | 50-68°C** | 30 sec | |
Extension | 72°C | 60 sec/kb | |
Final extension | 72°C | 3 min | 1 |
*Optimal PCR condition variesaccording to primers’ thermodynamic properties.
Post amplificationanalysis
Centrifuge PCR reaction mixture at 1000 × g for 1 ~ 3 minutes. Load only the clear supernatant of the PCRproducts for gel electrophoresis analysis.
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
Aptamer-based hot start PCR
Reversible enzyme inactivation
Omits extra enzyme activation step
Convenient for room temperature PCR set-up
High yield and specificity of target amplicons
Wide range of amplicon length (up to 10 kb)
High sensitivity (as low as 1 fg of plasmid)
High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye
5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
SMOBIO
SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。
产品列表:
斯莫比 | TP1000 | ExcelTaq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物,200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit,200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体,20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶,20,000单位,100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒,50 Rxn | 50 Rxn |
Protein 相关产品 | |||
斯莫比 | PM1500 | ExcelBand全蓝色常规范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1600 | ExcelBand全蓝色常规范围加蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围,250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程,250μlx 2 | 250微升×2 |
斯莫比 | PM5200 | ExcelBand 3色预染蛋白梯子,宽范围,250μlx 2 | 250微升×2 |
斯莫比 | PS1000 | FluoroStain蛋白荧光染色染料(红色,1000X),1毫升 | 1毫升 |
斯莫比 | PS1001 | FluoroStain蛋白荧光染色染料(红色,1000X),1 ml x 5 | 1毫升x 5 |
斯莫比 | PS2000 | FluoroStain蛋白荧光染色染料(金,100X),10毫升 | 10毫升 |
DNA 相关产品 | |||
斯莫比 | DM1100 | ExcelBand 50 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2100 | ExcelBand 100 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子,500μl | 500微升 |
斯莫比 | DM3100 | ExcelBand 1KB(0.25-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM3200 | ExcelBand 1KB Plus(0.1-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子,宽量程(最高25 kb),500μl | 500微升 |
斯莫比 | DM1160 | FluoroBand 50 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2160 | FluoroBand 100 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB(0.25-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus(0.1-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子,宽量程(最大25 kb),500μl | 500微升 |
斯莫比 | DL1000 | ExcelDye 6X DNA加载染料,橙色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL2000 | ExcelDye 6X DNA加载染料,绿色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料,蓝色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料,三色,5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料(绿色,6X),1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料(绿色,6X),1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
斯莫比 | VE0100 | B-BOXTM蓝光LED落射照明器,DC 12伏,0.72安培 | 1套 |
斯莫比 | VE0101 | 适配器,AC 100-240V,50/60 Hz,1.8 m电源线,1 /包 | |
斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板,1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜,1 /包 |