SMOBIO/[TP1260] ExcelTaq™ 5X Fluorescent PCR Master Mix, 200 Rxn/200 Rxn/TP1260,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[TP1260] ExcelTaq™ 5X Fluorescent PCR Master Mix, 200 Rxn/200 Rxn/TP1260

产品编号：TP1260

市场价：¥0.00

场地：美国(厂家直采)

产品分类：抗体类>二抗>抗人>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Description

The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.

Features

5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
For direct loading into electrophoresis analysis
DNA bands can be visualized directly under UV or 470 nm blue light illumination

Applications

Routine PCR
Colony PCR
High throughput PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling

Storage

Protected from light and avoid multiple freeze/thaw cycles4°C for 6 months-20°C for 24 months

Elongation capability

λDNA was amplified with ExcelTaq™ 5X Fluorescent PCR Master Mix. The λDNA was amplified with specific primers for amplifying different ranges. The pictures were captured under B-BOX™ blue light as seen in the left picture. While using UV light, the amplicons can be seen in the right picture (M: DM 3160).

Colony PCR

Colony PCR with ExcelTaq™ 5X Fluorescent PCR Master Dye Mix. The JM109 or modified JM109 was used for amplifying the genomic galactosidase Z gene. The pictures were captured under B-BOX™ blue light as seen in the left picture. While using UV light, the amplicons can be seen in the right picture (M: DM 3160).

Component

Volume

ExcelTaq™ 5X Fluorescent PCR Master Mix

2 x 1 ml

Storage

Protected from light and avoid multiple freeze/thaw cycles4°C for 6 months-20°C for 24 months

Manual

Manual_TP1260_ExcelTaq™ 5X Fluorescent PCR Master Mix

SDS

SDS_TP1260

Why is the size of the PCR product slightly different from prediction when used with TP1260 for PCR reaction?

It is due to variation of the fluorescent dye/DNA ratio. If less DNA was bond with too much fluorescent dye, the DNA would migrate slower in the electrophoresis and presented a larger size than expected. TP1260 therefore is not suitable for running such experiments needed to distinguish subtle differences.

Recommended PCR Condition

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

5X Fluorescence PCR Master Mix

10 µl

H2O

to 50 µl

Total volume

50 µl

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

*Optimal PCR condition varies according to primers’ thermodynamic properties.

B-BOX™ Blue Light LED Epi-illuminator

470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation

FluoroBand™ DNA Ladder series

Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive fluorescent dye

FluoroVue™ Nucleic Acid Gel Stain

Excellent for in-gel staining
Sensitivity up to 0.14 ng (DNA) or or 1 ng (total RNA)
A safe alternative to EtBr
Suitable for blue or UV light

High Fidelity PCR amplification

Amplification of target gene with HiFi™ DNA polymerase to minimize error rate.

[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)
[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.

Safe fluorescent dyes

[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

Blue-light illuminator

[VE0100] B-BOX™ Blue Light LED Epi-illuminator

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.

[CV1000] GetClone™ PCR Cloning Vector, 20 Rxn
[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.

[CK1000] Champion™ E. coli Transformation Kit

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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