SMOBIO/[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)/1 U/μl, 100 U/TF3000,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)/1 U/μl, 100 U/TF3000

产品编号：TF3000

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA修饰酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Description

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.

Features

5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate: 7 seconds/kb
High fidelity: 70 times higher than Taq polymerase
Generates blunt end amplicons
Vast elongation capability (up to 40 kb)
Thermo-stable for more than 10 hrs at 95°C

Storage

[TF3000] G-HiFi™ DNA Polymerase

-20°C for 24 months

Elongation capability

G-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 40 kb in length (M: DM5100).

Sensitivity

G-HiFi™ DNA Polymerase performs higher sensitivity for high GC content templates (GC: 71%) compare to high fidelity DNA Polymerase from Brand A (M: DM2000).

[TF3000] G-HiFi™ DNA Polymerase

Component

Volume

G-HiFi™ DNA Polymerase (1 U/μl)

100 μl

5X G-HiFi™ Buffer

1200 μl

dNTPs Mix (2 mM each)

600 μl

Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage

-20°C for 24 months

[TF3000] G-HiFi™ DNA Polymerase

Manual

Manual_TF3000_G-HiFi™ DNA Polymerase

SDS

SDS_TF3000

What are the differences between SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000)?

SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000) contain different high fidelity DNA polymerases. SMO-HiFi™ (TF1000) and G-HiFi™(TF3000) DNA polymerases are suitable for GC-rich templates that are difficult to amplify. Moreover G-HiFi™ (TF3000) is blended with elongation enhancer to perform quick and accurate PCR.

Recommended PCR Condition

[TF3000] G-HiFi™ DNA Polymerase

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM*

Reverse primer

0.1 – 0.5 µM*

5X G-HiFi™ Buffer

10 µl

dNTPs (2 mM each)

5 µl

G-HiFi™ DNA Polymerase

0.5 – 1 unit**

H2O

to 50 µl

Total volume

50 µl

*When amplifying products ≧ 10 kb in length, use primers at a final concentration of 0.1 μM each.

** When amplifying products ≦ 2 kb in length, use 0.5 unit of G-HiFi™ DNA Polymerase.

Recommended PCR Program

For ≦ 10 kb products

Select primers with a Tm value of ≧55°C. 20- to 25-mer primers are suitable, or those greater than 25-mer in length may provide optimal results.

Steps

Temp.

Time

Cycles

Template denature

98°C

2 min

Denature

98°C

10 sec

25-40

Annealing

50-68°C

15 sec

Extension

68°C

10-30 sec/kb

Final extension

68°C

1 min

*Optimal PCR condition varies according to primers’ thermodynamic properties.

For ≧ 10 kb products

Select primers with a Tm value of ≧ 65°C. 25- to 35-mer primers are suitable. Avoid high GC-content at the 3" end of each primer.

Steps

Temp.

Time

Cycles

Denature

98°C

10 sec

25-40

Extension

68°C

10-30 sec/kb

For GC-rich templates:

Steps

Temp.

Time

Cycles

Templatedenature

98°C

2 min

Denature

98°C

10 sec

25-40

Extension

68°C

10-30 sec/kb

AFEAP cloning: a precise and efficient method for large DNA sequence assembly

Fanli Zeng,1 Jinping Zang,1 Suhua Zhang,2 Zhimin Hao,1 Jingao Dong,corresponding author1 and Yibin Lincorresponding author3

BMC Biotechnol. 2017; 17: 81. Published online 2017 Nov 14. doi: 10.1186/s12896-017-0394-x

PMCID: PMC5686892

Gel electrophoresis

Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.

Safe fluorescent dyes

[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

Blue-light illuminator

[VE0100] B-BOX™ Blue Light LED Epi-illuminator

Ligation

Blund-end PCR amplicons can directly ligate with PCR cloning vector.

[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn

Transformation

Prepare competent cells with high efficiency and transform with time-saving protocol.

[CK1000] Champion™ E. coli Transformation Kit

Colony PCR

Analyze colonies with PCR master mix to save preparation time.

[TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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