Description
The SMO-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase with fidelity 70 times higher than Taq DNA polymerase during amplification, as well as very high elongation rate. Being highly thermostable, SMO-HiFi™ DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The SMO-HiFi™ DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase products.
Features
5"→3" DNA polymerase activity
3"→5" exonuclease activity (proofreading)
High reaction rate (up to 1 kb/10 second)
High fidelity, 70 times higher than Taq DNA polymerase
Blunt end amplicon
Thermo-stable-half life is more than 10 hrs at 95°C
Storage
[TF1000] SMO-HiFi™ DNA Polymerase
-20°C for 24 months
SMO-HiFi™ DNA Polymerase’s high processability enables reliable amplification of λDNA up to 12 kb in length (M: DM5100).
SMO-HiFi™ DNA Polymerase can amplify PCR products from as little as 1 fg of template DNA (M: DM2000).
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Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C for 24 months
[TF1000] SMO-HiFi™ DNA Polymerase
Manual
Manual_TF1000_SMO-HiFi™ DNA Polymerase
SDS
SDS_TF1000
SMO-HiFi™ (TF1000) and G-HiFi™ (TF3000) contain different high fidelity DNA polymerases. SMO-HiFi™ (TF1000) and G-HiFi™(TF3000) DNA polymerases are suitable for GC-rich templates that are difficult to amplify. Moreover G-HiFi™ (TF3000) is blended with elongation enhancer to perform quick and accurate PCR.
Recommended PCR Condition [TF1000] SMO-HiFi™ DNA Polymerase
Template | 1– 150 ng |
Forward primer | 0.1– 0.5 µM |
Reverse primer | 0.1– 0.5 µM |
10X HiFi™Buffer | 5µl |
MgSO4 (25 mM) | 2μl |
dNTPs (2 mM each) | 5µl |
SMO-HiFi™DNA Polymerase | 1µl (1 unit) |
DMSO | 5µl |
H2O | to50 µl |
Total volume | 50µl |
Recommended PCR Program
Steps | Temp. | Time | Cycles |
Template denature | 94°C | 2 min | 1 |
Denature | 94°C | 30 sec | 25-40 |
Annealing | 50-68°C | 30 sec | |
Extension | 68°C | 30 sec/kb | |
Final extension | 68°C | 1 min | 1 |
*Optimal PCR condition variesaccording to primers’ thermodynamic properties.
Gel electrophoresis
Staining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.
Safe fluorescent dyes
[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl
[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml
Blue-light illuminator
[VE0100] B-BOX™ Blue Light LED Epi-illuminator
Ligation
Blund-end PCR amplicons can directly ligate with PCR cloning vector.
[CV1100] GetClone™ PCR Cloning Vector II, 20 Rxn
Transformation
Prepare competent cells with high efficiency and transform with time-saving protocol.
[CK1000] Champion™ E. coli Transformation Kit
Colony PCR
Analyze colonies with PCR master mix to save preparation time.
[TP1100] ExcelTaq™ 5X PCR Master Mix, 200 Rxn
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix, 200 Rxn
SMOBIO
SMOBIO成立于2004年,公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物,范围从50bp到25kb。此外,SMOBIO提供各种DNA聚合酶和逆转录酶,可以针对不同的实验室应用进行优化。专注领域:蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年,SMOBIO Technology,Inc.迅速出现在生物技术行业。SMOBIO Technology,Inc.首先采用独特的发酵技术,旨在生产高质量的功能性食品成分。建立成功的分销网络后,我们将继续专注于高质量产品,并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来,SMOBIO Technology,Inc.已展示出非常可观的市场份额。客户满意的证据表明,SMOBIO Technology,Inc.竭尽全力生产高质量的产品。
产品列表:
斯莫比 | TP1000 | ExcelTaq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物,200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶,5 U /μl,500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit,200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液,200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体,20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶,20,000单位,100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒,50 Rxn | 50 Rxn |
Protein 相关产品 | |||
斯莫比 | PM1500 | ExcelBand全蓝色常规范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1600 | ExcelBand全蓝色常规范围加蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物,250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围,250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程,250μlx 2 | 250微升×2 |
斯莫比 | PM5200 | ExcelBand 3色预染蛋白梯子,宽范围,250μlx 2 | 250微升×2 |
斯莫比 | PS1000 | FluoroStain蛋白荧光染色染料(红色,1000X),1毫升 | 1毫升 |
斯莫比 | PS1001 | FluoroStain蛋白荧光染色染料(红色,1000X),1 ml x 5 | 1毫升x 5 |
斯莫比 | PS2000 | FluoroStain蛋白荧光染色染料(金,100X),10毫升 | 10毫升 |
DNA 相关产品 | |||
斯莫比 | DM1100 | ExcelBand 50 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2100 | ExcelBand 100 bp DNA梯子,500μl | 500微升 |
斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子,500μl | 500微升 |
斯莫比 | DM3100 | ExcelBand 1KB(0.25-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM3200 | ExcelBand 1KB Plus(0.1-10 kb)DNA梯子,500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子,宽量程(最高25 kb),500μl | 500微升 |
斯莫比 | DM1160 | FluoroBand 50 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2160 | FluoroBand 100 bp荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB(0.25-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus(0.1-10 kb)荧光DNA梯子,500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子,宽量程(最大25 kb),500μl | 500微升 |
斯莫比 | DL1000 | ExcelDye 6X DNA加载染料,橙色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL2000 | ExcelDye 6X DNA加载染料,绿色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料,蓝色,5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料,三色,5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料(绿色,6X),1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料(绿色,6X),1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料(绿色,10,000X),500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
斯莫比 | VE0100 | B-BOXTM蓝光LED落射照明器,DC 12伏,0.72安培 | 1套 |
斯莫比 | VE0101 | 适配器,AC 100-240V,50/60 Hz,1.8 m电源线,1 /包 | |
斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板,1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜,1 /包 |