SMOBIO/[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 Rxn/SYBR, no ROX), 200 Rxn</span> </li> </ol> </div> <div class=col-sm-3 mb8> <form method=get action=/shop/cate,SMOBIO,,SMOBIO,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：SMOBIO/[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 Rxn/SYBR, no ROX), 200 Rxn

产品编号：TQ1200

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细菌抗原>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： SMOBIO

公司：SMOBIO

公司分类：

商品介绍

Description

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program.

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

High Stability
Fast Hot Start
High Sensitivity
Low Background / High Specificity
Suitable for Fast Program
Smart Blue Contrast Dye

Storage

Protect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months

Fast Hot Start

The overlapped amplification curves from different hot start duration display fast activation of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The initial activation step was set as 2,5, and 10 minutes. TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) contains a special hot-start Taq polymerase that omits time-consuming initial activation step required by chemically modified hot start Taq polymerases (Brand A).

High Sensitivity

The amplification curves from different amounts of cDNA display high sensitivity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The sensitivity assay of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) was examined with serial diluted cDNA from 25 ng to 1.4 fg as templates. The equal intervals of amplification curves also displayed the high precision of TQ1200 in target quantification.

Low Background

The single peak of melting curve displays high specificity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The post-amplification melting-curve analysis was examined with 5 ng cDNA as template, abnormally high concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents.

High Stability

The overlapped amplification curves and melting curves from different storage conditions display high stability of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

Stability was analyzed via real-time PCR amplification with 1 ng cDNA as template, and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) stored at -20°C, 4°C, 22°C and 37°C for 30 days as reagents.

Suitable for Fast Program

To add a fourth column, reduce the size of thesethree columns using the right icon of each block.Then, duplicate one of the column to create a newone as a copy.

Smart Blue Contrast Dye

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Component

Volume

Cat. No

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

2 x 1 ml

TQ1200 (200 Rxn)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

5 x 1 ml

TQ1201 (500 Rxn)

Storage

Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months

Manual

Manual_TQ1200_ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

SDS

SDS_TQ1200

Flyer

SMOBIO’s Q-PCR Master Mix- Instrument Compatibility

ExcelTaq™ 2X Fast Q-PCR Master Mix

TaqMan® vs. SYBR® Green Chemistry

How can I choose ExcelTaq™ 2X Q-PCR Master Mix?

What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?

Product Name	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
Cat. No.	TQ1100	TQ1110	TQ1200	TQ1210	TQ2110
Detection chemistry	SYBR	SYBR	SYBR	SYBR	TaqMan
Blue contrast dye for best convenience	ü	ü	ü	ü	X
ROX dye for reference	X	ü	X	ü	ü
Time required for enzyme activation	10 min	10 min	2 min	2 min	10 min
qPCR program	Standard	Standard	Fast and Standard	Fast and Standard	Standard
Instrument Compatibility*
Applied Biosystems system:
5700, 7000, 7300, 7700, and 7900HT	Δ	V	Δ	V	V
StepOne, StepOnePlus	Δ	V	Δ	V	V
7500, 7500 Fast, QuantStudio, ViiA7	Δ	Δ	Δ	Δ	Δ
BioRad system:
CFX96 , CFX384	V	V	V	V	V
iQ5	V	V	V	V	V
Opticon, Opticon 2, Chromo 4	V	V	V	V	V
Cepheid system:
Smart Cycler	V	V	V	V	V
Eppendorf system:
Mastercycler ep realplex	V	V	V	V	V
Roche system:
LightCycler 480, Nano	V	V	V	V	V
QIAgen system:
Rotor-Gene™ 3000, 6000, Q	V	V	V	V	V
Agilent (Stratagene) system:
MX3000P, 3005P, 4000P	Δ	Δ	Δ	Δ	Δ
Illumina system:
Eco	V	V	V	V	V

*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.

V– Indicates the reagent is compatible to the instrument

Δ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.

Can you provide the results that using ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX, TQ1200) with different instruments?

Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore

Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y

PMCID: PMC6757040

Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore

Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328

Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells

Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

PMCID: PMC5522218

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver

Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

PMCID: PMC5706436

Loss of endogenous estrogen increases cardiac toxicity by doxorubicin

Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong

Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146

ExcelTaq™ 2X Q-PCR Master Mix

Product Name	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
Cat. No.	TQ1100	TQ1110	TQ1200	TQ1210	TQ2110
Detection chemistry	SYBR	SYBR	SYBR	SYBR	TaqMan
Blue contrast dye for best convenience	ü	ü	ü	ü	X
ROX dye for reference	X	ü	X	ü	ü
Time required for enzyme activation	10 min	10 min	2 min	2 min	10 min
qPCR program	Standard	Standard	Fast and Standard	Fast and Standard	Standard

品牌介绍

SMOBIO

SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。

2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。

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