![SMOBIO/[PS1000] FluoroStain™ Protein Fluorescent Staining Dye (Red, 1,000X), 1 ml/Red, 1,000X), 1 ml](images/SMOBIO/image.jpg)
Description
The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra (Fig. 2), i.e. LC-MS/MS, MALDI-TOF, etc.
Spectral Characteristics
When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm (Fig. 3). In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.
These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.
Storage
Protected from light -20°C for 24 months
Sensitivity of FluoroStain™
Comparison of FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) with Silver stain of a 2× serially diluted BSA sample.
Spectrum of FluoroStain™
Excitation and emission spectrum of FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×).
Compatibility to MASS analysis
Comparison of MALDI-TOF mass spectra of bovine serum albumin (BSA) stained with FluoroStain Protein Fluorescent Staining Dye (A) and with Coomassie Blue (B). BSA proteins are seperated on SDS-PAGE, stained with fluorescent dye or conventional Coomassie Blue, followed by trypsin digestion in gel, and then analyzed by MALDI-TOF.
Contents
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Manual
Manual_PS1000_FluoroStain™ Protein Fluorescent Staining Dye
SDS
SDS_PS1000
Can the SDS-PAGE gel stained with FluoroStain™ Protein Fluorescent Staining Dye (PS1000) be transferred to perform Western Blot? Will it affect the antibody binding when Western Blot is performed?
After using FluoroStain™ Protein Fluorescent Staining Dye (PS1000) to stain the gel, it is NOT recommended to perform Western Blot. The proteins in the SDS PAGE gel are fixed during staining with acidic solution, and thus preventing the transfer of proteins to NC or PVDF membrane.
When doing a follow up analysis using an SDS PAGE stained with FluoroStain™ Protein Fluorescent Staining Dye (PS1000), will there be any effect on mass analysis?
There will be no effect on mass analysis when following up with an SDS PAGE analysis using FluoroStain™ Protein Fluorescent Staining Dye (PS1000).
Is FluoroStain™ Protein Fluorescent Staining Dye (PS1000) able to stain a 2D gel?
The FluoroStain™ Protein Fluorescent Staining Dye (PS1000) can stain a 2D gel, which is quite sensitive. PS1000 is capable of achieving detection levels parallel to silver staining. The sample gel can be used for further MASS analysis.
Are there relevant document that support safety (ex. non-mutagenicity) of FluoroStain™ Protein Fluorescent Staining Dye (PS1000)?
FluoroStain™ Protein Fluorescent Staining Dye (PS1000) is proofed for their safety (non-mutagenicity) using Ames test (Figures below). However, it must be noted that since solvent may penetrate the skin, it is recommended that users wear gloves when using the fluorescent dyes.
Working Reagent Preparation
1:1000 dilution in 40% ethanol and 2% H3PO4Suggested volume of staining solution
Gel dimension(1 mm thick) | Gel volume | 1× staining solution |
9 cm × 7 cm | ≈ 6.5 ml | ≈ 100 ml |
13 cm × 9 cm | ≈ 12 ml | ≈ 180 ml |
16 cm × 16 cm | ≈ 26 ml | ≈ 390 ml |
26 cm × 23 cm | ≈ 60 ml | ≈ 900 ml |
B-BOX™ Blue Light LED Epi-illuminator
470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
470 nm long wavelength
Improved cloning efficiency
Compact, light-weight, and portable (less than 1 kg)
Adjustable and removable filter plate allows for gel cutting, visualization, and documentation
Q-PAGE™ Precast Gels
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
User-friendly gel cassette:
Numbered and framed wells for sample loading
Labeled warning sign and green tape as reminder
Enhanced gel performance:
Enhanced gel electrophoresis speed
Better band separation
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
SMOBIO
SMOBIO成立于2004年，公司在开发和制造分子生物学试剂和测试工具方面表现优异。其分子生物学试剂是市场上最受欢迎的一部分。提供的蛋白质标记物包含具有广谱预染蛋白的即用型稳定混合物，范围从50bp到25kb。此外，SMOBIO提供各种DNA聚合酶和逆转录酶，可以针对不同的实验室应用进行优化。专注领域：蛋白质标记、DNA梯度、PCR相关产品、克隆载体、荧光加载和染色染料、RT-PCR相关产品、Taq DNA聚合酶、Western Marker、dNTP等。
2004年，SMOBIO Technology，Inc.迅速出现在生物技术行业。SMOBIO Technology，Inc.首先采用独特的发酵技术，旨在生产高质量的功能性食品成分。建立成功的分销网络后，我们将继续专注于高质量产品，并开发了最先进的生物分子测试工具。我们的工具旨在通过提供高质量的DNA阶梯和蛋白质标记物来促进分子生物学和蛋白质科学研究。这些创新产品已受到业界和学术界人士的高度评价。自2008年以来，SMOBIO Technology，Inc.已展示出非常可观的市场份额。客户满意的证据表明，SMOBIO Technology，Inc.竭尽全力生产高质量的产品。
产品列表：
斯莫比 | TP1000 | ExcelTaq DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TK1000 | ExcelTaq Klen-Taq DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TF1000 | ExcelTaq SMO-HiFi DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TP1100 | ExcelTaq 5X PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | TP1200 | ExcelTaq 5X PCR主染料混合物，200 Rxn | 200 Rxn |
斯莫比 | TP1260 | ExcelTaq 5X荧光PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | TP2000 | ExcelTaq血液直接DNA聚合酶，5 U /μl，500 U x 1 | 500 U x 1 |
斯莫比 | TP2100 | ExcelTaq 5X Blood Direct PCR Master Mix Kit，200 Rxn | 200 Rxn |
斯莫比 | TQ1100 | ExcelTaq Q-PCR预混液，200 Rxn | 200 Rxn |
斯莫比 | CV1000 | GetCloneTMPCR克隆载体，20 Rxn | 200 Rxn |
斯莫比 | RP1000 | ExcelRT反转录酶，20,000单位，100μl100 Rxn | 100微升100 Rxn |
斯莫比 | RP1100 | ExcelRT一步式RT-PCR试剂盒，50 Rxn | 50 Rxn |
Protein 相关产品 | |||
斯莫比 | PM1500 | ExcelBand全蓝色常规范围蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM1600 | ExcelBand全蓝色常规范围加蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM1700 | ExcelBand全蓝色广谱蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2400 | ExcelBand粉色蓝色蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2500 | ExcelBand 3色常规蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2600 | ExcelBand 3色高范围蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM2700 | ExcelBand 3色广谱蛋白质标记物，250μlx 2 | 250微升×2 |
斯莫比 | PM5000 | ExcelBand 3色预染蛋白梯标准范围，250μlx 2 | 250微升×2 |
斯莫比 | PM5100 | ExcelBand 3色预染蛋白梯子高量程，250μlx 2 | 250微升×2 |
斯莫比 | PM5200 | ExcelBand 3色预染蛋白梯子，宽范围，250μlx 2 | 250微升×2 |
斯莫比 | PS1000 | FluoroStain蛋白荧光染色染料（红色，1000X），1毫升 | 1毫升 |
斯莫比 | PS1001 | FluoroStain蛋白荧光染色染料（红色，1000X），1 ml x 5 | 1毫升x 5 |
斯莫比 | PS2000 | FluoroStain蛋白荧光染色染料（金，100X），10毫升 | 10毫升 |
DNA 相关产品 | |||
斯莫比 | DM1100 | ExcelBand 50 bp DNA梯子，500μl | 500微升 |
斯莫比 | DM2100 | ExcelBand 100 bp DNA梯子，500μl | 500微升 |
斯莫比 | DM2300 | ExcelBand 100 bp + 3K DNA梯子，500μl | 500微升 |
斯莫比 | DM3100 | ExcelBand 1KB（0.25-10 kb）DNA梯子，500μl | 500微升 |
斯莫比 | DM3200 | ExcelBand 1KB Plus（0.1-10 kb）DNA梯子，500μl | 500微升 |
斯莫比 | DM4100 | ExcelBand XL 25 kb DNA梯子，宽量程（最高25 kb），500μl | 500微升 |
斯莫比 | DM1160 | FluoroBand 50 bp荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM2160 | FluoroBand 100 bp荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM2360 | FluoroBand 100 bp + 3K荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM3160 | FluoroBand 1KB（0.25-10 kb）荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM3260 | FluoroBand 1KB Plus（0.1-10 kb）荧光DNA梯子，500μl | 500微升 |
斯莫比 | DM4160 | FluoroBand XL 25 kb荧光DNA梯子，宽量程（最大25 kb），500μl | 500微升 |
斯莫比 | DL1000 | ExcelDye 6X DNA加载染料，橙色，5毫升x 2 | 5毫升x 2 |
斯莫比 | DL2000 | ExcelDye 6X DNA加载染料，绿色，5毫升x 2 | 5毫升x 2 |
斯莫比 | DL3000 | ExcelDye 6X DNA加载染料，蓝色，5毫升x 2 | 5毫升x 2 |
斯莫比 | DL4000 | ExcelDye 6X DNA加载染料，三色，5 ml x 2 | 5毫升x 2 |
斯莫比 | DL5000 | FluoroDye DNA荧光加载染料（绿色，6X），1毫升 | 1毫升 |
斯莫比 | DL5001 | FluoroDye DNA荧光加载染料（绿色，6X），1 ml x 5 | 1毫升x 5 |
斯莫比 | DS1000 | FluoroStain DNA荧光染色染料（绿色，10,000X），500μl | 500微升 |
斯莫比 | DS1001 | FluoroStain DNA荧光染色染料（绿色，10,000X），500μlx 5 | 500微升x 5 |
LED Light Box 切胶仪 | |||
斯莫比 | VE0100 | B-BOXTM蓝光LED落射照明器，DC 12伏，0.72安培 | 1套 |
斯莫比 | VE0101 | 适配器，AC 100-240V，50/60 Hz，1.8 m电源线，1 /包 | |
斯莫比 | VE0102 | 用于B-BOXTM的多角度滤板，1 /包 | |
斯莫比 | VE0103 | 用于B-BOXTM的琥珀色护目镜，1 /包 | |
![SMOBIO/[QP2320] Q-PAGE™ Bis-Tris Precast Gel (Mini, 15 wells, 12%), 10 gels/Mini, 15 wells, 12%), 10 gels</span>
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